Posted: Thu Jan 24, 2008 11:43 pm Post subject: Reply with quote
SAMPLE EXPERIMENT from KERVRAN'S BOOK
Experimental Demonstrations of the Existence of the Phenomenon of Biological Transmutation
(a) Research on Variations of Calcium and Potassium in Culture of Oats ~
(b) Research on K and Ca in Oats ~
(2) Simultaneous Investigation of Mg, K and Ca ~
(a) Experimental Protocol ~
__Oat seeds of the Flamingskrone variety (a blonde hybrid) with a 27.5 mg mean weight per seed were germinated in special vats covered with an indented plate with two seeds per indentation. The vats received double distilled water with a pH = 5.6.
__A preliminary control was accomplished by destroying the water and material of a vat to accomplish spectrophotometry of atomic absorption to verify that there was no measurable amount of calcium therein. The culture was accomplished without added fertilization.
__Germination took place in an enclosed space of about 70 x 40 x 30 cm of transparent plastic material out of contact with seeds and water. The enclosure was swept with air sent by an electric pump at a rate of about one liter/minute. This air passed through an air filter provided with one meter of hydrophilic cotton folded and compressed. Then it was muddled through four one-liter glass bottles, each filled with 750 ml of double distilled water, arranged in a series. The first was supplied with 30 ml of HCl to precipitate any trace of calcium dust which might have passed the filter. Then the air passed successively through two bottles with additives of NaHCO3 in order to neutralize any trace of acid drawn by the air from the preceding bottle. The fourth bottle contained only pure water.
__Thus, neither by air, nor by water, nor by material in contact with the germinating seeds was there any possibility of an introduction of Ca into the enclosure where there was an overpressure of approximately 3 mm water which made it impossible for any entry of ambient air contaminated by Ca.
__At the end of several weeks the plants developed from these seeds were gathered, dried, incinerated at 950 C, dissolved in hydrochloric acid and aliquot parts were analyzed by a number of methods in order to cross check results.
__At the same time there were analyzed some control segments of non-germinated seeds as nearly identical as possible to those which were germinated. All seeds utilized were calibrated and came from selected seedings furnished by the INRA and their germination rate was better than 95%. Nevertheless, each seed was hand-picked, the same for the control segments as for those which were germinated, in order to eliminate any abnormality of dimension (either too large or too small) or presenting a visible defect of form or of color, to have experimental segments as homogenous and as similar as possible.
__The essential purpose of the experiment, within the framework of verifying my studies, was to compare the quantity of Ca between seeds and plants in order to establish a balance sheet showing that germination of the oat --- a calcifugous plant --- in water with an acid pH will actually alter the quantity of Ca [after] a few weeks of growth in a calcium free environment.
MEAN PER SEED - MEAN PER PLANT - DIFFERENCE - RANGE: SEED ---------- RANGE: PLANT
K___0.113___________0.090_________-0.033_____+0.0072 to -0.0045_____+0.0119 to -0.0152
Ca__0.027___________0.059_________+0.032_____+0.0010 to -0.0015_____+0.0255 to -0.0128
Mg__0.031___________0.024_________-0.007_____+0.0059 to -0.0041_____+0.0053 to -0.0072
(d) Comparison of Variations in K and Ca ~
__In absolute value, K diminishes by 0.033 mg per unit while in absolute value, Ca increased by 0.032 mg per unit (between one seed and one plant derived from a similar seed).
__Thus there is a definite convergence between these two values, which allows us to conclude that the augmentation of Ca comes from reduction in K, from which we have the following statement:
__K 39/19 + H 1/1 >> Ca 40/20 + ~ 0.01 u.m.a.
__In different experiments this compensatory augmentation of Ca and reduction of K during germination of oats in an acid culture has been observed to approximately ± 4%, reflecting inevitable differences due to slight biological variations and experimental errors. Again, we can present these values as follows:
__in a seed: K + Ca = 0.140 mg
__in a plant: K + Ca = 0.139 mg
Indicating that the total amount of K + Ca does not change. or even:
__in the seed: K/Ca = 0.113 / 0.027 = 4.2 approximately;
__in the plant: K/Ca = 0.080 / 0.059 = 1.4 in excess,
which again reflects the reduction of K with respect to Ca. As K + Ca does not change, the increase of Ca can have no other origin than the diminution of K, which makes it useless to look for some other origin of Ca.
__The values of K and Ca, in both seeds and plants, as set forth above, are accepted without reservation because they confirm the mean measures supplied by other investigators using a variety of different analytic techniques.
__It is noted that in this experiment the relative increase of Ca is greater than 118%. The graph in Figure 3 clearly shows the reciprocal variations of K and Ca.
Last edited by lk on Thu Jan 24, 2008 11:52 pm; edited 2 times in total